Surface modification of titanium using BSA-Loaded chitosan and chitosan/gelatin polymers

Basit Öğe Kaydı
dc.authorid0000-0001-8170-7929
dc.contributor.authorÖnder, Sakipen_US
dc.date.accessioned2016-11-21T07:53:22Z
dc.date.available2016-11-21T07:53:22Z
dc.date.issued2016-10
dc.departmentIşık Üniversitesi, Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.departmentIşık University, Faculty of Engineering, Department of Biomedical Engineeringen_US
dc.description.abstractThe integration of titanium-based implants with the surrounding bone tissue needs to be improved to increase their service life. This work presents a surface modification technique to increase the osteointegration of titanium implants. The studies were conducted in three main steps: (i) surface modification of titanium using bovine serum albumin (BSA)-loaded chitosan and chitosan/gelatin polymers, (ii) studies of BSA release from these surfaces, (iii) effect of porosity and polymer composition on osteoblast cell proliferation. TiO2 and COOH groups were formed on titanium surfaces. Then, chitosan and chitosan (C)/gelatin (G) with various ratios (G:C = 0.5:1, 1:1, 1:0.5) were mixed with BSA and fixed to the surface via carbodiimide chemistry (EDC/NHS). After the immobilization process, samples were exposed to either air or freeze-drying. Characterization studies were conducted using Fourier transform-infrared spectroscopy and scanning electron microscopy. Finally, BSA release studies in phosphate-buffered saline (0.1 M, 37 °C) and cell (osteoblast) proliferation studies using MTS assay were conducted. BSA-loaded porous structures were obtained on chitosan- and chitosan/gelatin-containing surfaces after freeze-drying, while smooth surfaces were obtained after air-drying. The BSA release rate was directly correlated with increasing gelatin amount in the chitosan/gelatin coatings. MTS analysis was not conclusive because of the absorption properties of polymer coatings. However, absorbed color density in chitosan/gelatin (G:C = 1:1) polymers under freeze-drying conditions was more dominant, indicating better cell proliferation. This method may be used to release growth factors for controlled cell proliferation and differentiation or for the local delivery of antimicrobial drugs to prevent contamination during implementation in hard tissue applications.en_US
dc.description.versionPublisher's Versionen_US
dc.identifier.citationÖnder, S. (2016). Surface modification of titanium using BSA-loaded chitosan and Chitosan/Gelatin polymers. Journal of Medical and Biological Engineering, 36(5), 661-667. doi:10.1007/s40846-016-0172-6en_US
dc.identifier.doi10.1007/s40846-016-0172-6
dc.identifier.endpage667
dc.identifier.issn1609-0985
dc.identifier.issn2199-4757
dc.identifier.issue5
dc.identifier.scopus2-s2.0-84993965054
dc.identifier.scopusqualityQ3
dc.identifier.startpage661
dc.identifier.urihttps://hdl.handle.net/11729/1148
dc.identifier.urihttp://dx.doi.org/10.1007/s40846-016-0172-6
dc.identifier.volume36
dc.identifier.wosWOS:000387227600006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakScience Citation Index Expanded (SCI-EXPANDED)en_US
dc.institutionauthorÖnder, Sakipen_US
dc.institutionauthorid0000-0001-8170-7929
dc.language.isoenen_US
dc.peerreviewedYesen_US
dc.publicationstatusPublisheden_US
dc.publisherSpringer Berlin Heidelbergen_US
dc.relation.ispartofJournal of Medical and Biological Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBovine serum albuminen_US
dc.subjectChitosanen_US
dc.subjectGelatinen_US
dc.subjectHard tissue implantsen_US
dc.subjectOsteoblasten_US
dc.subjectTitaniumen_US
dc.subjectBody fluidsen_US
dc.subjectCell proliferationen_US
dc.subjectCellsen_US
dc.subjectChitinen_US
dc.subjectCoatingsen_US
dc.subjectCytologyen_US
dc.subjectDryingen_US
dc.subjectFourier transform infrared spectroscopyen_US
dc.subjectLow temperature dryingen_US
dc.subjectMammalsen_US
dc.subjectOsteoblastsen_US
dc.subjectPlastic coatingsen_US
dc.subjectPolymersen_US
dc.subjectPorosityen_US
dc.subjectScanning electron microscopyen_US
dc.subjectSurface plasmon resonanceen_US
dc.subjectSurface treatmenten_US
dc.subjectTissueen_US
dc.subjectBovine serum albuminsen_US
dc.subjectCarbodiimide chemistryen_US
dc.subjectCharacterization studiesen_US
dc.subjectPhosphate-buffered salinesen_US
dc.subjectSurface modification techniquesen_US
dc.subjectTitanium-based implantsen_US
dc.titleSurface modification of titanium using BSA-Loaded chitosan and chitosan/gelatin polymersen_US
dc.typeArticleen_US
dspace.entity.typePublication

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
1148.pdf
Boyut:
1.21 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Publisher's Version
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.71 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Makale Koleksiyonu | Biyomedikal Mühendisliği Bölümü
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu