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dc.contributor.authorÖnder, Sakipen_US
dc.date.accessioned2016-11-21T07:53:22Z
dc.date.available2016-11-21T07:53:22Z
dc.date.issued2016-10
dc.identifier.citationÖnder, S. (2016). Surface modification of titanium using BSA-loaded chitosan and Chitosan/Gelatin polymers. Journal of Medical and Biological Engineering, 36(5), 661-667. doi:10.1007/s40846-016-0172-6en_US
dc.identifier.issn1609-0985
dc.identifier.issn2199-4757
dc.identifier.urihttps://hdl.handle.net/11729/1148
dc.identifier.urihttp://dx.doi.org/10.1007/s40846-016-0172-6
dc.description.abstractThe integration of titanium-based implants with the surrounding bone tissue needs to be improved to increase their service life. This work presents a surface modification technique to increase the osteointegration of titanium implants. The studies were conducted in three main steps: (i) surface modification of titanium using bovine serum albumin (BSA)-loaded chitosan and chitosan/gelatin polymers, (ii) studies of BSA release from these surfaces, (iii) effect of porosity and polymer composition on osteoblast cell proliferation. TiO2 and COOH groups were formed on titanium surfaces. Then, chitosan and chitosan (C)/gelatin (G) with various ratios (G:C = 0.5:1, 1:1, 1:0.5) were mixed with BSA and fixed to the surface via carbodiimide chemistry (EDC/NHS). After the immobilization process, samples were exposed to either air or freeze-drying. Characterization studies were conducted using Fourier transform-infrared spectroscopy and scanning electron microscopy. Finally, BSA release studies in phosphate-buffered saline (0.1 M, 37 °C) and cell (osteoblast) proliferation studies using MTS assay were conducted. BSA-loaded porous structures were obtained on chitosan- and chitosan/gelatin-containing surfaces after freeze-drying, while smooth surfaces were obtained after air-drying. The BSA release rate was directly correlated with increasing gelatin amount in the chitosan/gelatin coatings. MTS analysis was not conclusive because of the absorption properties of polymer coatings. However, absorbed color density in chitosan/gelatin (G:C = 1:1) polymers under freeze-drying conditions was more dominant, indicating better cell proliferation. This method may be used to release growth factors for controlled cell proliferation and differentiation or for the local delivery of antimicrobial drugs to prevent contamination during implementation in hard tissue applications.en_US
dc.language.isoengen_US
dc.publisherSpringer Berlin Heidelbergen_US
dc.relation.isversionof10.1007/s40846-016-0172-6
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBovine serum albuminen_US
dc.subjectChitosanen_US
dc.subjectGelatinen_US
dc.subjectHard tissue implantsen_US
dc.subjectOsteoblasten_US
dc.subjectTitaniumen_US
dc.subjectBody fluidsen_US
dc.subjectCell proliferationen_US
dc.subjectCellsen_US
dc.subjectChitinen_US
dc.subjectCoatingsen_US
dc.subjectCytologyen_US
dc.subjectDryingen_US
dc.subjectFourier transform infrared spectroscopyen_US
dc.subjectLow temperature dryingen_US
dc.subjectMammalsen_US
dc.subjectOsteoblastsen_US
dc.subjectPlastic coatingsen_US
dc.subjectPolymersen_US
dc.subjectPorosityen_US
dc.subjectScanning electron microscopyen_US
dc.subjectSurface plasmon resonanceen_US
dc.subjectSurface treatmenten_US
dc.subjectTissueen_US
dc.subjectBovine serum albuminsen_US
dc.subjectCarbodiimide chemistryen_US
dc.subjectCharacterization studiesen_US
dc.subjectPhosphate-buffered salinesen_US
dc.subjectSurface modification techniquesen_US
dc.subjectTitanium-based implantsen_US
dc.titleSurface modification of titanium using BSA-Loaded chitosan and chitosan/gelatin polymersen_US
dc.typearticleen_US
dc.description.versionPublisher's Versionen_US
dc.relation.journalJournal of Medical and Biological Engineeringen_US
dc.contributor.departmentIşık Üniversitesi, Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümüen_US
dc.contributor.departmentIşık University, Faculty of Engineering, Department of Biomedical Engineeringen_US
dc.contributor.authorID0000-0001-8170-7929
dc.identifier.volume36
dc.identifier.issue5
dc.identifier.startpage661
dc.identifier.endpage667
dc.peerreviewedYesen_US
dc.publicationstatusPublisheden_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorÖnder, Sakipen_US
dc.relation.indexWOSen_US
dc.relation.indexScopusen_US
dc.relation.indexScience Citation Index Expanded (SCI-EXPANDED)en_US
dc.description.qualityQ4
dc.description.wosidWOS:000387227600006


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